The use of chilled and frozen semen in a fixed terminology artificial insemination process in Wagyu steers
Keywords:
Cryopreservation, frozen sperm, heifers, artificial intelligenceAbstract
The objective of this research was to determine the effect of semen cryopreservation on reproductive efficiency in a beef heifer herd. Ninety-seven Wagyu crossbred heifers and cows were used. The first fraction (SC frozen semen) was diluted 1:8 by adjusting the inseminant dose to 25 x 106 spz in 0.5 ml straws. For freezing, the standard freezing method was used, freezing in liquid nitrogen vapors. A gynecological examination was performed on the selected heifers: In addition, Artificial Insemination was performed at a fixed time 52 hours after the implants were removed. The results show the existence of a significance of (P<0.05) with an association between frozen/thawed and refrigerated semen and it is statistically significant. Also, a significance of (P<0.05) was obtained between frozen/thawed and refrigerated semen on sperm membrane integrity and is statistically significant. The findings demonstrate that spermatozoa from each of the heifers evaluated resisted cryopreservation differently, therefore the structural and functional integrity of heifers may be negatively affected by cryogenic damage, which could affect the fertilizing ability of semen samples intended for artificial intelligence (AI).
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